*/Disease Information (sort of like the Intro to your Mini Research Write up): Malaria is one of the most predominate diseases in the world. Around 3.5 million people are have a moderate to high potential of getting the disease. It mainly is in Africa, India, and in central South America because of the presence of the Anopheles gambiae mosquito that serves as a carrier to disease causing Plasmodium flaciparum. One way the Plasmodium flaciparum produces proteins is by using the amino acids of the hemoglobin on erthyrocytes. This causes the oxygen transport that takes place through the blood cells to be hindered. Symptoms of malaria are nausea, vomit, diarrhia, and anemia.
Is it a monomer or multimer as biological unit? (make prediction at__http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html__): 2 amino acid chains and 2 ligands in ASU Complex of proteins?: Yes Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism): 0.5 __http://www.tdrtargets.org/targets/view?gene_id=4612__ Structure-activity relationships at the 5-position of thiolactomycin: an intact (5R)-isoprene unit is required for activity against the condensing enzymes from Mycobacterium tuberculosis and Escherichia coli. (2006). J Med Chem 49: 159-71.
Expression Information (has it been expressed in bacterial cells): E.coli Purification Method: Expressed in E. coli with an N-terminal polyhistidine tag and subsequently purified by metal chelate and size exclusion chromatography. __http://aac.asm.org/content/46/5/1310__
Image of protein (PyMol with features delineated and shown separately):
c2.PNG
*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHIAAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVAT
Format for Individual Target pages (copy this list to new Target page and then fill in for your target):
*Target (protein/gene name):
BETA-KETOACYL-ACP SYNTHASE I
__http://www.tdrtargets.org/targets/view?gene_id=4612__
*NCBI Gene # or RefSeq#:
3885996
__http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=full_report&list_uids=3885996__
*Protein ID (NP or XP #) or Wolbachia#:
XP_966246.2
__http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=full_report&list_uids=3885996__
*Organism (including strain):
Plasmodium falciparum 3D7
Etiologic Risk Group (see link below): Group B disease__http://ideas.health.vic.gov.au/bluebook/malaria.asp__ http://www.tulane.edu/~wiser/malaria/fv.html
*/Disease Information (sort of like the Intro to your Mini Research Write up):
Malaria is one of the most predominate diseases in the world. Around 3.5 million people are have a moderate to high potential of getting the disease. It mainly is in Africa, India, and in central South America because of the presence of the Anopheles gambiae mosquito that serves as a carrier to disease causing Plasmodium flaciparum. One way the Plasmodium flaciparum produces proteins is by using the amino acids of the hemoglobin on erthyrocytes. This causes the oxygen transport that takes place through the blood cells to be hindered. Symptoms of malaria are nausea, vomit, diarrhia, and anemia.
Link to TDR Targets page (if present):
__http://www.tdrtargets.org/targets/view?gene_id=4612__
Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)
__http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=full_report&list_uids=3885996__
Essentiality of this protein: Catalyzes the biosynthesis of fatty acids.
http://www.rcsb.org/pdb/explore/explore.do?structureId=1HN9
Is it a monomer or multimer as biological unit? (make prediction at__http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html__):
2 amino acid chains and 2 ligands in ASU
Complex of proteins?: Yes
Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):
0.5
__http://www.tdrtargets.org/targets/view?gene_id=4612__
Structure-activity relationships at the 5-position of thiolactomycin: an intact (5R)-isoprene unit is required for activity against the condensing enzymes from Mycobacterium tuberculosis and Escherichia coli. (2006).
J Med Chem 49: 159-71.
*EC#:
2.3.1.41
Link to BRENDA EC# page:
__http://www.brenda-enzymes.org/enzyme.php?ecno=2.3.1.41&Suchword=&organism%5B%5D=Plasmodium+falciparum&show_tm=0__
-- Show screenshot of BRENDA enzyme mechanism schematic
Capture.PNG
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):__http://www.ncbi.nlm.nih.gov/pubmed/18437316__
-- link to Sigma (or other company) page for assay (see Sigma links below)
-- -or link (or citation) to paper that contains assay information
-- links to assay reagents (substrates) pages.
__http://download-v2.springer.com/static/pdf/329/chp%253A10.1007%252F978-1-59745-246-5_16.pdf?token2=exp=1429408833~acl=%2Fstatic%2Fpdf%2F329%2Fchp%25253A10.1007%25252F978-1-59745-246-5_16.pdf*~hmac=c4d17d97c2d4acc7414488569db5814860f50aefe7afdc1a5f0e7ab04e9f40d9__
--- List cost and quantity of substrate reagents, supplier, and catalog #
Substrate reagent: Lauroyl CoA
Quantity: 5MG,
Catolog #: L2659,
Cost: $154.00,
Supplier: Sigma
Structure (PDB or Homology model)
-- PDB # or closest PDB entry if using homology model:
1HN9
Current Inhibitors:
Cerulenin
http://www.drugbank.ca/biodb/bio_entities/BE0000801
Expression Information (has it been expressed in bacterial cells): E.coli
Purification Method:
Expressed in E. coli with an N-terminal polyhistidine tag and subsequently purified by metal chelate and size exclusion chromatography.
__http://aac.asm.org/content/46/5/1310__
Image of protein (PyMol with features delineated and shown separately):
*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHIAAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVAT
TSATHAFPSAACQIQSMLGIKGCPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVTELAHIVDETLAANNLDRSQLDW
LVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTSAASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF
*length of your protein in Amino Acids
317 residues
Molecular Weight of your protein in kiloDaltons using the __Expasy ProtParam__ website
69441.6
Molar Extinction coefficient of your protein at 280 nm wavelength:
626262
TMpred graph Image (__http://www.ch.embnet.org/software/TMPRED_form.html__). Input your amino acid sequence to it.
__http://www.ch.embnet.org/cgi-bin/TMPRED_form_parser__
*CDS Gene Sequence (paste as text only):
atgtataccaaaattattggcaccggcagctatctgccggaacaggtgcgcaccaacgcg gatctggaaaaaatggtggataccagcgatgaatggattgtgacccgcaccggcattcgc gaacgccatattgcggcgccgaacgaaaccgtgagcaccatgggctttgaagcggcgacc cgcgcgattgaaatggcgggcattgaaaaagatcagattggcctgattgtggtggcgacc accagcgcgacccatgcgtttccgagcgcggcgtgccagattcagagcatgctgggcatt aaaggctgcccggcgtttgatgtggcggcggcgtgcgcgggctttacctatgcgctgagc gtggcggatcagtatgtgaaaagcggcgcggtgaaatatgcgctggtggtgggcagcgat gtgctggcgcgcacctgcgatccgaccgatcgcggcaccattattatttttggcgatggc gcgggcgcggcggtgctggcggcgagcgaagaaccgggcattattagcacccatctgcat gcggatggcagctatggcgaactgctgaccctgccgaacgcggatcgcgtgaacccggaa aacagcattcatctgaccatggcgggcaacgaagtgtttaaagtggcggtgaccgaactg gcgcatattgtggatgaaaccctggcggcgaacaacctggatcgcagccagctggattgg ctggtgccgcatcaggcgaacctgcgcattattagcgcgaccgcgaaaaaactgggcatg agcatggataacgtggtggtgaccctggatcgccatggcaacaccagcgcggcgagcgtg ccgtgcgcgctggatgaagcggtgcgcgatggccgcattaaaccgggccagctggtgctg
ctggaagcgtttggcggcggctttacctggggcagcgcgctggtgcgcttt
*GC% Content for gene: 60.3%
*CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):
TACCAACGCTGACCTGGAAAAAATGGTTGACACCTCTGACGAATGGATCG 100
TTACCCGTACCGGTATCCGTGAACGTCACATCGCTGCTCCGAACGAAACC 150
GTTTCTACCATGGGTTTCGAAGCTGCTACCCGTGCTATCGAAATGGCTGG 200
TATCGAAAAAGACCAGATCGGTCTGATCGTTGTTGCTACCACCTCTGCTA 250
CCCACGCTTTCCCGTCTGCTGCTTGCCAGATCCAGTCTATGCTGGGTATC 300
AAAGGTTGCCCGGCTTTCGACGTTGCTGCTGCTTGCGCTGGTTTCACCTA 350
CGCTCTGTCTGTTGCTGACCAGTACGTTAAATCTGGTGCTGTTAAATACG 400
CTCTGGTTGTTGGTTCTGACGTTCTGGCTCGTACCTGCGACCCGACCGAC 450
CGTGGTACCATCATCATCTTCGGTGACGGTGCTGGTGCTGCTGTTCTGGC 500
TGCTTCTGAAGAACCGGGTATCATCTCTACCCACCTGCACGCTGACGGTT 550
CTTACGGTGAACTGCTGACCCTGCCGAACGCTGACCGTGTTAACCCGGAA 600
AACTCTATCCACCTGACCATGGCTGGTAACGAAGTTTTCAAAGTTGCTGT 650
TACCGAACTGGCTCACATCGTTGACGAAACCCTGGCTGCTAACAACCTGG 700
ACCGTTCTCAGCTGGACTGGCTGGTTCCGCACCAGGCTAACCTGCGTATC 750
ATCTCTGCTACCGCTAAAAAACTGGGTATGTCTATGGACAACGTTGTTGT 800
TACCCTGGACCGTCACGGTAACACCTCTGCTGCTTCTGTTCCGTGCGCTC 850
TGGACGAAGCTGTTCGTGACGGTCGTATCAAACCGGGTCAGCTGGTTCTG 900
CTGGAAGCTTTCGGTGGTGGTTTCACCTGGGGTTCTGCTCTGGTTCGTTT 950
C
*GC% Content for gene (codon optimized):
53.3%